Thp-1 phagocytosis assay protocol
WebJan 13, 2024 · Allergen recognition and processing by antigen presenting cells is essential for the sensitization step of food allergy. Macrophages and dendritic cells are both … Webderived macrophages, or cell lines like RAW 264.7 or THP-1) INTRODUCTION About This Assay Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with …
Thp-1 phagocytosis assay protocol
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Webconcentration on signal-to-noise ratio of THP-1 chemotaxis at 20, 25, and 30 minutes post-incubation with 25 nM MCP-1. Figure 6. THP-1 cells were seeded at 100,000 cells/well on … WebMar 14, 2024 · THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the …
WebJan 10, 2024 · THP-1 cells were seeded in 2-well chamber slides, differentiated and treated as indicated. Macrophages were then assessed for phagocytic activity using the … Web2 days ago · An increase in exposure of β-(1,3)-glucan and chitin on the cell surface was also observed through flow cytometry. Interestingly, we observed a decrease in the phagocytosis rate when the Δcdc50 mutant was co-incubated with THP-1 macrophages. The Δcdc50 mutant also exhibited weakened virulence in nematode survival tests.
WebJan 10, 2024 · Figure 1. Microtubule staining of THP-1-derived macrophages. (a) Resting stage macrophages were fixed with a new protocol and immunostained with an anti-α-tubulin antibody (red), phalloidin (green), and DAPI (blue). Confocal optical sections of the upper half (Z = 0.9–4.2 µm) and lower half (Z = 0.0–0.9 µm) were stacked. WebHuman monocytic cell line THP-1 was purchased from cell bank of Chinese Academy of Sciences (Shanghai, ... Phagocytosis assays. After M2-polarized macrophage cells …
WebDec 21, 2024 · THP-1 macrophages were exposed to Nx NPs for 48 h and cytokine secretion was measured in supernatants by a Luminex multiplex bead assay using an in-house …
WebJan 1, 2024 · The detailed protocols were described in Supplementary Methods. 2.7. WST-8 assay for detection of macrophage metabolic activity. BMDMs cultured with L929 … colin hewitson krhWebLoss of DJ-1 resulted in an increased ROS production enhancing bacterial phagocytosis and killing. Further, DJ-1 deficiency altered the balance between autophagy and phagocytosis … dr oglander crestwood moWebBut sometimes cells deattach during the polarization process (48h). Here is the protocol, which I am using: 1. Seeding THP-1 cells (1,3x10 6 / well) in 6-well plates (2ml RPMI … colin higham facebookcolin hickey githubWebOct 1, 2024 · For plate differentiated THP-1 cells, 1 × 10 6 cells were added per tube. FACS Wash solution (2 mL) was added to each tube before centrifugation at 500 ×g. 2.5. … colin hewett real estateWebAug 8, 2015 · THP-1 polarization into pro-inflammatory M1 macrophages. The classical protocol for M1 polarization is to incubate macrophages in the presence of IFN-γ alone or … colin hibbsWebOct 31, 2024 · Opsonic phagocytosis is an important host immune effector mechanism against blood- stage Plasmodium falciparum 1,2,3,4.The intracellular parasite produces … dr oglesby ashdown