Webb27 jan. 2014 · Reference strains of C. botulinum were grown in Wilkins Chalgren broth (WB) , incubated anaerobically at 35°C for 48 hrs, and then harvested by low speed centrifugation. Pellets were resuspended in 1 ml of distilled water, and chromosomal DNA extracted using the QIAamp DNA Mini Kit (Qiagen Inc., Valencia, CA), according to the … WebbIan Chalgren Book designer, comic book designer, corporate designer. Enjoys cheese, meat, and fire. 5 d Denunciar esta publicação Denunciar Denunciar. Voltar ...
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