WebNational Center for Biotechnology Information Web17 Jan 2014 · However, p53 phosphorylation at Ser37 was not affected by any agent combination in either cell line . In addition, PARP cleavage, which is an indicator of apoptosis, increased in cells subjected to treatment with both agents in Bel-7402, Huh7 and Hep3B (p53 +/+) cells . These data suggested that MKT-077 may improve the anti-tumor …
A common Gain of function of p53 cancer mutants in inducing …
Human hepatocytes were prepared from liver biopsies obtained from two patients (one female and one man, aged 43 ± 6.0 years) submitted to surgical resection for liver tumors after obtaining patients’ written consent. The study protocol was approved by the Ethical Committee of the Institution. The … See more Sorafenib (FS10808), Regorafenib (FR16116), Cabozantinib (FD59688), and Lenvatinib (FC75063) were obtained from Carbosynth Limited (Berkshire, UK). Drugs were solved in dimethyl sulfoxide as stock solution (100 mM). See more Cell proliferation was assessed in 3D and 2D cultured cells. Non-trypan blue-stained viable cells were quantified in trypsin-treated spheroids, as well as Ki67-positive cells were … See more Mitochondrial respiration in intact adhered cells was assessed with the XFp Cell Mito Stress Test Kit in a XFp Extracellular Flux Analyzer (Seahorse Bioscience, Billerica, Massachusetts, … See more Cell death was assessed either in trypsin-isolated cells with the measurement of trypan blue-stained non-viable cells in spheroids, or TUNEL … See more WebTo assess the molecular basis for p53-mediated Nestin repression in human HCC cell lines, we explored factors that influence Nestin expression in human HCC cell lines with … adips guideline gtt
Novel CDK9 inhibitor oroxylin A promotes wild-type P53 stability …
Web29 Nov 2024 · Among them, the p53 mutation is found in ~60% of colorectal cancers, and a majority of mutations are missense-type at ‘hot spots’, suggesting an oncogenic role of mutant p53 by ‘gain-of-function’ mechanisms. Mouse model studies have shown that one of these missense-type mutations, p53 R270H (corresponding to human R273H), causes ... Web13 Nov 2024 · Huh7 only have a single copy of mutated p53 coding DNA locus (Y220C/del), and MHCC-97H have a heterozygous p53 coding DNA locus with one wild type copy and one mutant copy (R249S). As both MHCC-97H and Huh7 have mutant version of p53 (Fig. 2d), we would wonder if PPPDE1 is a regulator of both mutant and wild type p53. WebThe tumor suppressor p53 plays a key role in regulating cell cycle arrest, DNA repair, apoptosis, and cell senescence and is inactivated either by mutation or up-regulation of proteins such as MDM2 or MDMX in virtually all human cancers (1–4).The function of p53 may be impaired by mutation of residues involved directly in DNA binding (contact … adips gestational diabetes