WebSep 27, 2024 · Dynamic mass redistribution (Epic) assay. Vero cells were seeded at a cell density of 5 × 10 3 cells/well in a 384-well fibronectin-coated Epic plate (Corning #5042) and grown overnight at 37°C in 5% CO 2. On the day of assay, medium was aspirated and replaced with 40 μl/well assay buffer (HBSS in 20 mM HEPES) and equilibrated at RT for 1 …
Applying label-free dynamic mass redistribution assay for …
WebAn example is the Epic® optical-based biosensor that relies on dynamic mass redistribution (DMR) for detection. So far, DMR assays have been mostly used to study G protein-coupled receptor (GPCR) pharmacology. Here, we demonstrate the utility of this assay for investigating ligand-gated ion channel receptors. WebDec 3, 2024 · Dynamic mass redistribution assay. Confluent cells were subcultured using trypsin/EDTA and used for experiments. Cells were seeded at a density of 20 000 cells/well in 30 μL into fibronectin‐coated Enspire™‐LC 384‐wells plates and cultured for 20 hours to form a confluent monolayer. On the day of the experiment, cells were manually ... tina turner iconic looks
Dynamic Mass Redistribution Assay Decodes ... - ScienceDirect
Web2.4 Dynamic mass redistribution assays All DMR assays were performed using an Epic BT system (Corning, NY, USA). Cells were directly seeded in Epic 384-Well Cell Assay Microplates and cultured for 24 h to form a monolayer in the cell culture medium. A er being washed, the cells were main-tained with 30 mL assay buffer for 1 h in the Epic system. WebStem cells hold great potential in drug discovery and development. However, challenges remain to quantitatively measure the functions of stem cells and their differentiated products. Here, we applied fluorescent imaging, quantitative real-time PCR, and label-free dynamic mass redistribution (DMR) assays to characterize the differentiation process of … WebNov 1, 2024 · Dynamic mass redistribution label-free assay The DMR assay was performed using an Epic Benchtop System from Corning® (Corning, New York, NY; USA) and … tina turner i don\u0027t wanna fight youtube