Webto soluble denatured extraction, (S1000 Assay) and place into a screw capped collagen round bottom 2 ml digestion tube (as supplied in the S2000 Assay Kit). ... unbound … WebNov 20, 2024 · Total soluble collagen was measured in the culture supernatant by the Sircol Soluble Collagen Assay (Biocolor, S1000). Briefly, 50 µL of cell culture supernatant was adjusted to 100 µL. The sample concentration was adjusted according to the reaction results to make it within the range of the standard curve. Then, 1 mL of Sircol dye was added ...
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WebSupplier: ILEX LIFE SCIENCES LLC S1000. The Biocolor Sircol™ Soluble Collagen Assay is a quantitative assay for measurement of both acid-soluble and pepsin-soluble … WebDec 31, 2024 · The Sircol soluble collagen assay (Biocolor, S1000) and Fastin Elastin assay kits (Biocolor, F2000) were used. HDF were cultured in a 6-well culture plate and treated with MSC- CM for 72 h. After performing the assays according to the manufacturer’s instructions, collagen and elastin concentrations were measured using a microplate … WebThe patented † BioSampler ® is a result of SKC's work with leading scientists at the University of Cincinnati. Allows use of non-evaporating collection liquids. Patented … bwa water additives